Off-target analysis into the NEB-step 1 and HeLa phone traces

Off-target analysis into the NEB-step 1 and HeLa phone traces Inverse nested PCR is actually did playing with genomic DNA regarding twenty six puromycin-unwilling NEB-1 clones that happen to be obtained from a few separate transfections (A) Schematic attracting proving notice-ligation off PstI digested genomic DNA comprising incorporated pattP4X-PGKssPuro target vector. Ranking out-of related primers …

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